In Vitro Pharmacological Investigations Of Aqueous Fraction Of Coccinia Cordifolia Leaves

Transcript

In vitro pharmacological investigations of aqueous fraction of Coccinia cordifolia leaves 1.Introduction: 1.1 Medicinal plants; 1.2 Medicinal plants of Bangladesh; 1.3 Vernacular names of Coccinia cordifolia; 1.4 Taxonomy of C. cordifolia; 1.5 Cucurbitaceae family; 1.5.1 Species of Cucurbitaceae available in Bangladesh; 1.6 Coccinia cordifolia; 1.7 Synonyms of Coccinia cordifolia; 1.8 Distribution of Coccinia cordifolia; 1.9 Habitant of Coccinia cordifolia; 1.10 Morphology of plant; 1.10.1 Stem of C. cordifolia; 1.10.2 Leaf of C. cordifolia; 1.10.3 Flower of C. cordifolia; 1.10.4 Fruit of C. cordifolia; 1.10.5 Seed of C. cordifolia; 1.10.6 Root of C. cordifolia; 1.11 Reproduction and dispersal of Coccinia cordifolia; 1.12 Vegetative propagation of Coccinia cordifolia; 1.13 Nutritional value of Coccinia cordifolia; 1.14 Local uses of C. cordifolia in Bangladesh; 1.15 Toxicology of C. cordifolia 2. Literature Review: 2.1 Phytochemical constituents; 2.2 Pharmacological properties; 2.2.1 Anti-diabetic and hypoglycemic activity; 2.2.1.1 In vivo study on human model; 2.2.1.2 In vivo study on animal model; 2.2.2 Hypolipidemic and antidyslipidemic activity; 2.2.3 Anti-inflammatory activity; 2.2.4 Analgesic and antipyretic activity; 2.2.5 Anti-bacterial and anti-fungal activity; 2.2.6 Anthelmintic activity; 2.2.7 Cytotoxic, antitumer and pesticidal activity; 2.2.8 Antioxidant activity; 2.2.9 Chemoprotective activity; 2.2.10 Antiulcer and cytoprotective activity; 2.2.11 Anti-hepatotoxic activity; 2.2.12 Fertility inducing activity; 2.2.13 Larvicidal, repellent and egg hatching inhibition property; 2.2.14 Antilithiatic activity Rationale and objective of the work 3. Methods and Materials: 3.1 Collection and preparation of plant material; 3.2 Extraction of the plant material; 3.3 Preparation of mother solution; 3.4 Partition of mother solution; 3.4.1 Partition with n-hexane; 3.4.2 Partition with carbon tetrachloride; 3.4.3 Partition with chloroform; 3.4.4 Partition with ethyl acetate; 3.4.5 Collection of aqueous Fraction; 3.5 Antioxidant activity; 3.5.1 DPPH (1,1-diphenyl-2-picrylhydrazyl) radical test; 3.5.1.1 Principle; 3.5.1.2 Apparatus and reagents; 3.5.1.3 Procedure; 3.5.2 Total phenolic content; 3.5.2.1 Principle; 3.5.2.2 Apparatus and reagents; 3.5.2.3 Procedure; 3.5.3 Total flavonoid content; 3.5.3.1 Principle; 3.5.3.2 Apparatus and reagents; 3.5.3.2 Procedure; 3.6 Brine shrimp lethality bioassay; 3.6.1 Principle; 3.6.2 Apparatus and reagents; 3.6.3 Procedure; 3.6.3.1 Preparation of sea water; 3.6.3.2 Hatching of brine shrimp; 3.6.3.3 Preparation of test solutions; 3.6.3.5 Counting of nauplii; 3.7 Antimicrobial activity by disc diffusion method; 3.7.1 Principle; 3.7.2 Apparatus and reagents; 3.7.2.1 Materials; 3.7.2.2 Test sample of Coccinia cordifolia; 3.7.2.3 Test organisms; 3.7.3 Procedure; 3.7.3.1 Preparation of the medium; 3.7.3.2 Sterilization procedure; 3.7.3.3 Preparation of test plate; 3.7.3.4 Preparation of discs; 3.7.3.5 Preparation of test sample; 3.7.3.6 Application of test samples; 3.7.3.7 Diffusion and incubation; 3.7.3.8 Determination of antimicrobial activity by measuring the zone of inhibition 4. Results and Discussion: 4.1 Antioxidant test results; 4.1.1 DPPH test results; 4.1.1.1 Preparation of standard curve4.1.1.2 Preparation of aqueous fraction curve; 4.1.1.3 Discussion; 4.1.2 Total phenol content test results; 4.1.2.1 Preparation of standard curve; 4.1.2.2 Total phenolic content present in aqueous fraction; 4.1.2.3 Discussion; 4.1.3 Total flavonoid content result; 4.1.3.1 Preparation of standard curve; 4.1.3.2 Total flavonoid content present in aqueous fraction; 4.1.3.3 Discussion; 4.2 Brine shrimp lethality bio-assay result; 4.2.1 Preparation of standard curve; 4.2.2 Preparation of aqueous fraction curve; 4.2.3 Discussion; 4.3 Antibacterial test results; 4.3.1 Zone of inhibition of standard and aqueous fraction; 4.3.2 Discussion 5. Conclusion: 5.1 Conclusion 6. Reference